| 基于自动磁珠转运的转基因蛋白Cry1Ab检测 |
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| 引用本文： | 李冬阳,樊凯,吴坚,应义斌. 基于自动磁珠转运的转基因蛋白Cry1Ab检测[J]. 分析化学, 2011, 0(9): 1318-1322 |
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| 作者姓名： | 李冬阳 樊凯 吴坚 应义斌 |
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| 作者单位： | 浙江大学生物系统工程与食品科学学院; |
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| 基金项目： | 国家科技专项(No.2009ZX08012-004B)资助 |
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| 摘 要： | 基于自动磁珠转运,建立了转基因蛋白Cry1Ab免疫检测的新方法.利用水热法制备了粒径约400 nm的纳米磁球,并进行电镜表征,通过溶胶法对磁球表面进行氨基修饰,采用戊二醛偶联对磁珠实现抗体包被,在核酸提取仪中进行酶联免疫反应,采用分光光度法进行检测.本方法对转基因蛋白Cry1Ab的检出限低于1 μg/L,与商品化酶联免...
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| 关 键 词： | 转基因作物 磁分离 Cry1Ab蛋白 磁珠转运 免疫检测 |
Magnetic Beads Transfer Based Assay for Cry1 Ab Protein |
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| LI Dong-Yang,FAN Kai,WU Jian,YING Yi-Bin. Magnetic Beads Transfer Based Assay for Cry1 Ab Protein[J]. Chinese Journal of Analytical Chemistry, 2011, 0(9): 1318-1322 |
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| Authors: | LI Dong-Yang FAN Kai WU Jian YING Yi-Bin |
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| Affiliation: | LI Dong-Yang,FAN Kai,WU Jian,YING Yi-Bin (College of Biosystem Engineering and Food Science,Zhejiang University,Hangzhou 310029) |
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| Abstract: | A novel method for the immunoassay of genetically modified organisms(GMOs) protein Cry1Ab was proposed.First the nano-magnetic beads with the diameters around 400 nm were prepared by hydrothermal method.Then,the prepared magnetic beads were modified with NH2 group using sol-gel approach.The antibodies were conjugated on the surface of magnetic beads through bi-functional agent glutaraldehyde.The immunoassay was carried out in the nucleic acid extractor which is commercial available.In this proposed method,t... |
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| Keywords: | Genetically modified organisms Magnetic separation Cry1Ab protein Magnetic beads transfer Immunoassay |
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