Development of a novel label-free amperometric immunosensor for the detection of okadaic acid |
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Authors: | Akhtar Hayat Lise BarthelmebsAudrey Sassolas Jean-Louis Marty |
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Institution: | Univ. Perpignan Via Domitia, Institut de Modélisation et d’Analyse en Géo-Environnement et Santé, EA 4218, F-66860, Perpignan, France |
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Abstract: | Okadaic acid (OA), a lipophilic phycotoxin is mainly produced by toxigenic dinoflagellates. The need to develop high performing methods for OA analysis able to improve the traditional ones is evident. In this work, a novel experimental methodology for label-free detection of OA was developed. Protein G magnetic beads (protein-G-MBs) modified gold electrode was used to immobilize anti-OA monoclonal antibody (anti-OA-MAb). Preliminary, colorimetric tests were performed in order to validate protein-G-MBs and anti-OA-MAb reaction. Electrochemical detection was carried out by differential pulse voltammetry in ferri/ferrocyanide solution. The limit of detection value obtained (0.5 μg L−1) validated the developed electrochemical immunosensor as a promising tool for routine use. The matrix effect and the recovery rate were also assessed with real samples, showing a good percentage of recovery. |
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Keywords: | Okadaic acid Protein G magnetic beads Label-free immunosensor Amperometric immunosensor Mussel extract |
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