A simple protocol for Matrix Assisted Laser Desorption Ionization- time of flight-mass spectrometry (MALDI-TOF-MS) analysis of lipids and proteins in single microsamples of paintings |
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| Authors: | Inez D van der Werf Cosima D Calvano Francesco Palmisano Luigia Sabbatini |
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| Institution: | 1. Dipartimento di Chimica, “Laboratorio di ricerca per la diagnostica dei Beni Culturali”, Università degli Studi di Bari Aldo Moro, Via Orabona, 4, 70126 Bari, Italy;2. Centro Interdipartimentale S.M.A.R.T., “Laboratorio di ricerca per la diagnostica dei Beni Culturali”, Università degli Studi di Bari Aldo Moro, Via Orabona, 4, 70126 Bari, Italy;3. Centro Interdipartimentale, “Laboratorio di ricerca per la diagnostica dei Beni Culturali”, Università degli Studi di Bari Aldo Moro, Via Orabona, 4, 70126 Bari, Italy |
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| Abstract: | A simple protocol, based on Bligh–Dyer (BD) extraction followed by MALDI-TOF-MS analysis, for fast identification of paint binders in single microsamples is proposed. For the first time it is demonstrated that the BD method is effective for the simultaneous extraction of lipids and proteins from complex, and atypical matrices, such as pigmented paint layers. The protocol makes use of an alternative denaturing anionic detergent (RapiGest™) in order to improve efficiency of protein digestion and purification step. Detection of various lipid classes, such as triacylglycerols (TAGs) and phospholipids (PLs), and their oxidation by-products was accomplished, whereas proteins could be identified by peptide mass fingerprinting. The effect of pigments on ageing of lipids and proteins was also investigated. |
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| Keywords: | Bligh&ndash Dyer Conservation science Paint binder MALDI-TOF-MS Proteomics Lipidomics |
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