Towards an integrated biosensor array for simultaneous and rapid multi-analysis of endocrine disrupting chemicals |
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Authors: | Viviana Scognamiglio Italo Pezzotti Gianni Pezzotti Juan Cano Ivano Manfredonia Katia Buonasera Fabiana Arduini Danila Moscone Giuseppe Palleschi Maria Teresa Giardi |
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Institution: | 1. IC-CNR Istituto di Cristallografia, AdR1 Dipartimento Agroalimentare – Via Salaria Km 29.3 00015, Rome, Italy;2. Biosensor S.r.l. – Via degli Olmetti 44 00060 Formello, Rome, Italy;3. Università di Roma Tor Vergata, Dipartimento di Scienze e Tecnologie Chimiche – Via della Ricerca Scientifica 00133, Rome, Italy |
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Abstract: | In this paper we propose the construction and application of a portable multi-purpose biosensor array for the simultaneous detection of a wide range of endocrine disruptor chemicals (EDCs), based on the recognition operated by various enzymes and microorganisms. The developed biosensor combines both electrochemical and optical transduction systems, in order to increase the number of chemical species which can be monitored. Considering to the maximum residue level (MRL) of contaminants established by the European Commission, the biosensor system was able to detect most of the chemicals analysed with very high sensitivity. In particular, atrazine and diuron were detected with a limit of detection of 0.5 nM, with an RSD% less than 5%; paraoxon and chlorpyrifos were revealed with a detection of 5 μM and 4.5 μM, respectively, with an RSD% less than 6%; catechol and bisphenol A were identified with a limit of detection of 1 μM and 35 μM respectively, with an RSD% less than 5%. |
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Keywords: | Endocrine disrupting chemicals Multi-array Biosensor Amperometric Optical Integrated biosensing system |
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