完整大豆叶片中自由基对的相互作用

本工作对完整大豆叶片直接地进行了电子自旋共振谱的测量.通过求解薛定格方程,给出IP-700+的数值解析表达式,该式与ESR实验曲线符合的很好.从而证实电子自旋极化理论对大豆叶片中光诱导信号I动力学和线型分析的适用性,以及得到A1是叶绿素A二聚物的判断.

RADICAL PAIR INTERACTION IN INTACT SOYBEAN LEAVES

Light induced electron spin polarization is an important consequence of charge separation in the primary photoreactions of photosynthesis.The informations on the structure and constituent of the primary photoreactions and their interaction could be obtained. In this paper, P-700+and A1-centers were studied in PSI of the intact soybean leaves with ESR techniques and curve fits at room temperature. For ESR measurements, the leaves were irradiated with ultraviolet light(337.1nm) and cw He-Ne laser. When orientation of the intact soybean leaves was changed in the magnetic field, we observed the dependence of electron spin polarization on the orientation.Our choice of the leaves for this study was based on the current model of the PSI reaction center organization, which may be represented as P-700A1XFdBFdA. According to the radical pair theory, we could write an approximate spin Hamiltonian of P-700+and A1-radical pair asH=βH0·((gD·SD+gD·SA)-J(2SD·SA+1/2)+ΣAi(D)Ii(D)·SD+ΣAi(A)Ii(A)SAand the experimental ESR intensity Ip-700+of P-700+as a function of field position H is given by IP-700+(H)=Σ-(pi)e(H-Hi)2/δ2.