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Immobilization of Biomolecules on NH3, H2/NH3 Plasma-Treated Nitrocellulose Films |
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| Authors: | Tatoulian M. Arefi-Khonsari F. Amouroux J. Rejeb S. Ben Martel A. Durand N. Fischer Lawrence J.F. Le Goffic F. |
| Affiliation: | (1) Laboratoire de Génie des Procédés Plasma, Université Pierre et Marie Curie, ENSCP, 11 rue Pierre et Marie Curie, 75231 Paris Cedex 05, France;(2) Laboratoire de Biotechnologies de l'Environnement, EP 105 CNRS, Ecole Nationale Supérieure de Chimie de Paris, 11 rue Pierre et Marie Curie, 75231 Paris Cedex 05, France;(3) Food Research Division, Food Directorate, Bureau of Chemical Safety, Health Protection Branch, Health Canada, PL 2203D Ottawa, Ontario, Canada |
| Abstract: | Detectors with covalently immobilized bioactive compounds able to interact with selected analytes are needed for various kinds of biosensors. Protein macromolecules can be immobilized covalently on surfaces with appropriate functional groups such as amine groups. Low pressure plasmas of NH3 and NH3/H2 mixtures were used to incorporate a maximum of 2.4 amine functions per nm2 of porous surface under specific conditions. A mechanism of the functionalization process was proposed using on-line emission spectroscopy and mass spectrometry analysis to monitor the reaction process. The physico-chemical modifications created on the plasma-treated material were investigated by surface analytical techniques such as X-ray photoelectron spectroscopy (XPS) and Scanning Electron Microscopy (SEM). |
| Keywords: | Biosensors physico-chemical modifications X-ray photoelectron spectroscopy scanning electron microscopy |
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