Spectrophotometric determination of deacetylation degree of chitinous materials dissolved in phosphoric acid |
| |
| Authors: | Hsiao Hsien-Yi Tsai Chih-Cheng Chen Suming Hsieh Bo-Chuan Chen Richie L C |
| |
| Institution: | Biosensor Laboratory, Department of Bio-Industrial Mechatronics Engineering, College of Bioresources and Agriculture, National Taiwan University, 136 Chou-Shan Road, Taipei, Taiwan 106, R.O.C. |
| |
| Abstract: | A simple spectrophotometric method is proposed for determining deacetylation degrees (DD) of chitinous materials using phosphoric acid as the UV-transparent solvent system. Calibrating by the extinction coefficients (A(210)) of D-glucosamine and N-acetyl-D-glucosamine, DD values (24-88%) were computed numerically. The results correlated well (R(2) = 0.9805, n = 50) with those obtained by solid-state (13)C NMR. Comparison of the results obtained by the proposed UV method and solid-state (13)C NMR. |
| |
| Keywords: | chitin chitosan deacetylation degree NMR UV‐vis spectroscopy |
| 本文献已被 PubMed 等数据库收录！ |
|
