| RP-HPLC测定八角枫药材中的水杨苷 |
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| 引用本文： | 段红,翟科峰,高贵珍,曹稳根,薛国辉.RP-HPLC测定八角枫药材中的水杨苷[J].光谱实验室,2012,29(2):1065-1068. |
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| 作者姓名： | 段红 翟科峰 高贵珍 曹稳根 薛国辉 |
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| 作者单位： | 1. 宿州学院特色种植业苗种生产工程技术研究中心,安徽省宿州市汴河中路71号,234000
2. 宿州学院化学与生命科学学院,安徽省宿州市汴河中路71号,234000 |
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| 基金项目： | 安徽省教育厅自然科学研究项目(KJ2011B182);宿州学院特色种植业苗种生产工程技术研究中心开放课题(2011YKF19),宿州学院产学研合作项目(2011cxy01);安徽省高等学校省级忧秀青年人才基金(2012SQRL200) |
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| 摘 要： | 建立反相高效液相色谱法测定八角枫药材中水杨苷含量。采用Shim-pack vp-ODS C18(150mm×4.6mm,5μm),柱温为25℃;以乙腈-水(7∶93,V/V)为流动相,流速为1.0mL.min-1;检测波长为269nm。水杨苷在16.02—80.10μg.mL-1范围内线性关系良好,线性方程为y=5070.1x+4069.1,r=0.9994,平均回收率为99.53%,RSD=1.19%。该方法快速简单,可用于八角枫药材的质量控制。
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| 关 键 词： | 八角枫 水杨苷 反相高效液相色谱法 |
Determination of Salicin in Alangium Chinese(Lour.) Harms Medicinal Materials by RP-HPLC |
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| DUAN Hong
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ZHAI Ke-Feng
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GAO Gui-Zhena
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CAO Wen-Gena
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XUE Guo-Huia.Determination of Salicin in Alangium Chinese(Lour.) Harms Medicinal Materials by RP-HPLC[J].Chinese Journal of Spectroscopy Laboratory,2012,29(2):1065-1068. |
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| Authors: | DUAN Hong ZHAI Ke-Feng GAO Gui-Zhena CAO Wen-Gena XUE Guo-Huia |
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| Institution: | DUAN Hong ZHAI Ke-Feng GAO Gui-Zhena CAO Wen-Gena XUE Guo-Huia(Engineering Research Center of Special Farm Seed Production,Suzhou University,Suzhou,Anhui 234000,P.R.China)a(College of Chemistry & Life Science,Suzhou University,Suzhou,Anhui 234000,P.R.China) |
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| Abstract: | The method for the determination of salicin in Alangium Chinese(Lour.) Harms medicinal materials was established by high performance liquid chromatography(HPLC).The determination was carried out over a Shim-pack vp-ODS C18 column(150mm×4.6mm,5μm) at the column temperature of 25℃.The mobile phase was consisted of acetonitrile(7%)-water(93%) with flow rate of 1.0mL·min-1,and detection wavelength was 269 nm.There was a good linear relation for salicin in the range of 16.02—80.10μg·mL-1.The linear equation was y=5070.1x+4069.1 with correlation coefficient of 0.9994,and the average recovery was 99.53% with RSD of 1.19%.The method is fast and simple,that can be applied to controlling quality of Alangium Chinese(Lour.) Harms medicinal materials. |
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| Keywords: | Alangium Chinese(Lour ) Harms Salicin RP-HPLC |
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