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| 反相高效液相色谱法测定维格列汀 | |
| 引用本文： | 马廉举,文棘,胡煮,黄一可,梁定骅,彭俊,胡湘南. 反相高效液相色谱法测定维格列汀[J]. 光谱实验室, 2012, 29(4): 2555-2558 |
| 作者姓名： | 马廉举 文棘 胡煮 黄一可 梁定骅 彭俊 胡湘南 |
| 作者单位： | 1. 重庆医科大学药学实验教学中心 重庆市渝中区医学院路1号重庆医科大学383号信箱 400016 2. 重庆医科大学药学实验教学中心 重庆市渝中区医学院路1号重庆医科大学383号信箱 400016;重庆医科大学药学院 重庆市渝中区医学院路1号重庆医科大学380号信箱 400016 3. 重庆医科大学药学院 重庆市渝中区医学院路1号重庆医科大学380号信箱 400016 |
| 摘 要： | 建立反相高效液相色谱法测定维格列汀的含量。采用Venusil MP C18色谱柱（250mm×4.6mm,5μm）,流动相为磷酸盐缓冲溶液(称取20mmol·L-1磷酸二氢钾,用85%浓磷酸调至pH3.0)-乙腈（88:12,V/V）,检测波长为210nm,流速为1.0mL·min-1,柱温为25℃。维格列汀在5—500μg·mL-1范围内与峰面积呈良好的线性关系（r=0.9999）,维格列汀的平均回收率为102.0%,RSD为2.65%。方法准确、简便,适用于维格列汀的定量分析。 |
| 关 键 词： | 维格列汀 反相高效液相色谱法 含量 |
Determination of Vildagliptin by RP-HPLC |
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| MA Lian-Ju , WEN Ji , HU Zhu , HUANG Yi-Ke , LIANG Ding-Hua , PENG Jun , HU Xiang-Nan. Determination of Vildagliptin by RP-HPLC[J]. Chinese Journal of Spectroscopy Laboratory, 2012, 29(4): 2555-2558 | |
| Authors: | MA Lian-Ju WEN Ji HU Zhu HUANG Yi-Ke LIANG Ding-Hua PENG Jun HU Xiang-Nan |
| Affiliation: | Ma Lian-Jua Wen Ji(a,b) Hu Zhu(a,b) Huang Yi-Ke(a,b) LIANG Ding-Hua(a,b) Peng Junb Hu Xiang-Nanb a(Pharmaceutical Experimental Teaching Center,Chongqing Medical University,Chongqing 400016,P.R.China) b(College of pharmacy,Chongqing Medical University,Chongqing 400016,P.R.China) |
| Abstract: | The content of vildagliptin was determined by RP-HPLC.The separation conditions were as follows:column was Venusil MP C18（250mm×4.6mm,5μm）,mobile phase was phosphate buffer(pH of the weighed 20mmol·L-1 monopotassium phosphate was adjusted to 3.0 by 85% phosphoric acid)-acetonitrile（88:12,V/V）,detection wavelength was 210nm,flow rate was 1.0 mL·min-1 and the column temperature was 25℃.The vildagliptin had the good linear relationship with peak area in the rang of 5-500μg·mL-1（r=0.9999）,and the average recovery was 102.0% with RSD of 2.65%.This method is reliable,accurate and suitable,and can be applied to quantitative analysis of vildagliptin. |
| Keywords: | Vildagliptin RP-HPLC Content |
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