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| 叶酸-壳聚糖偶联物的制备及对人体血红细胞的影响 | |
| 引用本文： | 蔡树山,刘宗华,陈莉莉,张渊明,薛巍. 叶酸-壳聚糖偶联物的制备及对人体血红细胞的影响[J]. 光谱实验室, 2012, 29(3): 1702-1706 |
| 作者姓名： | 蔡树山 刘宗华 陈莉莉 张渊明 薛巍 |
| 作者单位： | 1. 暨南大学化学系 广州市黄埔大道西601号 510632;中山市东区中学 中山市孙文东路齐乐路3号 528403 2. 暨南大学生命与健康工程研究院 广州市黄埔大道西601号 510632 3. 暨南大学化学系 广州市黄埔大道西601号 510632 |
| 基金项目： | 广东省自然科学基金，广州市科技计划项目 |
| 摘 要： | 通过叶酸活性酯与壳聚糖的氨基反应,制备出叶酸-壳聚糖偶联物;经红外光谱、核磁共振表征该偶联物的化学结构,紫外分光光度法测定每个壳聚糖上叶酸的接枝量。通过溶血实验、扫描电镜、原子力显微镜观察比较了壳聚糖、叶酸-壳聚糖对人体红细胞的影响。结论:成功制备出叶酸-壳聚糖偶联物;与壳聚糖相比,接枝叶酸的壳聚糖对血红细胞的形貌没有改变。 |
| 关 键 词： | 叶酸-壳聚糖偶联物 人体红细胞 形貌 |
Preparation of Folate-Chitosan Conjugate and Its Influence on Human Red Blood Cells |
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| CAI Shu-Shan , LIU Zong-Hua , CHEN Li-Li , ZHANG Yuan-Ming , XUE Wei. Preparation of Folate-Chitosan Conjugate and Its Influence on Human Red Blood Cells[J]. Chinese Journal of Spectroscopy Laboratory, 2012, 29(3): 1702-1706 | |
| Authors: | CAI Shu-Shan LIU Zong-Hua CHEN Li-Li ZHANG Yuan-Ming XUE Wei |
| Affiliation: | b a(Department of Chemistry,Jinan University,Guangzhou 510632,P.R.China)b(Institutes of Life and Health Engineering,Jinan University,Guangzhou 510632,P.R.China)c(Dongqu Middle School,Zhongshan,Guangdong 528403,P.R.China) |
| Abstract: | The folate-chitosan conjugate was prepared by the reaction of the activated folate ester with the amine group of chitosan.The chemical structure of folate-chitosan conjugate was characterized by infrared spectroscopy and magnetic nuclear resonance,and grafted quantity of folate on each chitosan was determined by UV spectrophotometry.The effects of chitosan and folate-chitosan conjugate on human red blood cells were studied and compared by hemolysis test,scanning electron microscopy and atomic force microscopy.The results showed that the folate-chitosan conjugate was prepared successfully,and compared with chitosan,the folate-chitosan conjugate did not change the morphology of the red blood cells. |
| Keywords: | Folate-Chitosan Conjugate Human Red Blood Cells Morphology |
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