| 铽-茜素红作为光散射探针测定洛美沙星的共振瑞利散射光谱及其应用 |
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| 引用本文： | 王媚,韩权,杨晓慧.铽-茜素红作为光散射探针测定洛美沙星的共振瑞利散射光谱及其应用[J].光谱实验室,2012,29(3):1477-1481. |
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| 作者姓名： | 王媚 韩权 杨晓慧 |
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| 作者单位： | 1. 陕西中医学院药学院 陕西省咸阳市世纪大道中段 712046
2. 西安文理学院化学与化学工程系 西安市太白南路168号 710065 |
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| 摘 要： | 提出了共振瑞利散射光谱法测定洛美沙星的新方法。在pH 5.0—6.0的HAc-NaAc缓冲溶液中,铽()与洛美沙星(Lomefloxacin,LOM)抗生素能形成阳离子配合物,它可进一步通过静电引力和疏水作用与茜素红(Alizarin Red,AR)阴离子反应形成1:2:1(Tb3+:LOM:AR)三元离子缔合物,此时将出现新的共振瑞利散射(Resonance Rayleigh Scattering,RRS)光谱,其两个散射峰分别位于370nm和590nm处。在370nm处,一定浓度的洛美沙星与散射增强(ΔI)成正比,其线性范围和检出限(3σ)分别是0.004—3.52μg.mL-1和14.3 ng.mL-1。该方法简单、快速、具有良好的选择性和重复性,可用于不同体液中洛美沙星药物的测定。文中还对反应机理作了讨论。
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| 关 键 词： | 洛美沙星 铽(Ⅲ) 茜素红 共振瑞利散射 |
Resonance Rayleigh Scattering Spectrum in Determination of Lomefloxacin with Tb3+-Alizarin Red as Light Scattering Probe and Its Application |
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| WANG Mei
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HAN Quan
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YANG Xiao-Hui.Resonance Rayleigh Scattering Spectrum in Determination of Lomefloxacin with Tb3+-Alizarin Red as Light Scattering Probe and Its Application[J].Chinese Journal of Spectroscopy Laboratory,2012,29(3):1477-1481. |
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| Authors: | WANG Mei HAN Quan YANG Xiao-Hui |
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| Institution: | a(College of Pharmacy,Shaanxi University of Chinese Mdeicine,Xianyang,Shaanxi 712046,P.R.China)a(Department of Chemistry and Chemical Engineering,Xi′an University of Arts and Science,Xi′an 710065,P.R.China) |
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| Abstract: | A novel method for the determination of lomefloxacin(LOM) by resonance Rayleigh scattering(RRS)spectrometry was developed.In pH 5.0—6.0 HAc-NaAc buffer solution,lomefloxacin can react with Tb3+ to form cation chelates,which further bond with alizarin red(AR) anion to form ternary ion-association complexes(Tb3+∶LOM∶AR) at the ratio of 1∶2∶1 by electrostatic attraction and hydrophobic interaction,that resulted in a new RRS spectrum.The two scattering peaks located at 370nm and 590nm.The RRS intensity enhancement(ΔI) was proportional to the certain concentration of LOM in the range of 0.004—3.52μg·mL-1,and the detection limit was 14.3ng·mL-1.The method is simple,rapid with the good selectivity and repeatability,that is applied to the determination of LOM in the different body fluid.The reaction mechanism was also investigated. |
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| Keywords: | Lomefloxacin Tb3+ Alizarin Red Resonance Rayleigh Scattering |
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