Synthesis of Codon-optimized Human Interleukin-18 Gene by Combination of Chemical and Enzymatic Method |
| |
| Authors: | GAO Chao-hui SHI Xiao-yue HOU Xin-tong MENG Qing-fan Zhang Ying-jiu TENG Li-rong |
| |
| Institution: | [1]College of Life Science, Jilin University, Changehun 130012, P. R. China [2]Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, Jilin University, Changehun 130021, P. R. China [3]Changehun Diseases Control and Prevention Center, Changehun 130033, P. R. China [4]College of Animal Science and Veterinary Medicine, Jilin University, Changehun 130062, P. R. China |
| |
| Abstract: | According to the amino acid sequence and codon preference of E,coli,the human interleukin-18(IL-18)gene was optimized to avoid the rare codons,The total length of the synthesized gene is 571 bp;18 oligonucleotides,DNA fragments were designed and synthesized by the phosphoramidite four-step chemical method,The whole DNAsequence was synthesized by a one-step total gene synthesis method,and then inserted in pUC18 vector,Five positive clones identified by blue-white colony screening were sent to Shanghai Sangon Biological Engineering Technology and Service Co.,Ltd,for sequencing,The sequencing result shows that one clone contained the complete correct gene in all the five positive clones. |
| |
| Keywords: | Human IL-18 Gene synthesis One-step method Oligodeoxynucleotide synthesis |
| 本文献已被 维普 万方数据 ScienceDirect 等数据库收录！ |
|
