Electrochemical Biosensors Based on Enzymatic Reactor of Silver Solid Amalgam Powder for Measurements in Flow Systems |
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| Authors: | Oksana Josypčuk Jiří Barek Bohdan Josypčuk |
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| Affiliation: | 1. J. Heyrovsky Institute of Physical Chemistry of AS CR, v.v.i., Department of Biomimetic Electrochemistry, Dolejskova 3, 182?23 Prague, Czech Republic tel: +420?266?053?895;2. Charles University in Prague, Faculty of Science, University research center UNCE “Supramolecular chemistry”, Department of Analytical Chemistry, UNESCO Laboratory of Environmental Electrochemistry, Albertov 6, CZ‐128?43 Prague 2, Czech Republic |
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| Abstract: | An enzymatic reactor based on silver solid amalgam powder was suggested as the main part of biosensors in flow systems for the first time. Biosensors were tested with following enzymes: ascorbate oxidase, glucose oxidase, catalase, tyrosinase and laccase. The current response of each biosensor was optimized with respect to the detection potential, flow rate, the injection and reactor volume. Relative standard deviation for detection with the studied enzymes was found to be in the range of 0.81–2.1 %. The biosensor with the ascorbate oxidase reactor was used for determination of ascorbic acid in the vitamin tablets Celaskon. |
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| Keywords: | Electrochemical biosensors Flow systems Amperometry Amalgam powder Enzymatic reactor |
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