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Poly‐ε‐Lysine Modified Nanocarbon Film Electrodes for LPS Detection |
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| Authors: | Dai Kato Atsumu Oda Mutsuo Tanaka Seiichiro Iijima Tomoyuki Kamata Masami Todokoro Yasuo Yoshimi Osamu Niwa |
| Affiliation: | 1. National Institute of Advanced Industrial Science and Technology (AIST), 1‐1‐1 Higashi, Tsukuba, Ibaraki 305‐8566, Japan;2. Graduate School of Pure and Applied Sciences, University of Tsukuba, 1‐1‐1 Tennodai, Tsukuba, 305‐8573, Japan;3. JNC Corporation, 5‐1, Okawa, Kanazawa‐ku, Yokohama, 236‐8605, Japan;4. Shibaura Institute of Technology, 3‐7‐5 Toyosu, Koto‐ku, Tokyo 135‐8548, Japan |
| Abstract: | We developed an electrochemical system for detecting lipopolysaccharide (LPS) that uses an ultraflat nanocarbon film electrode modified with poly‐ε‐lysine with a high affinity to LPS. LPS was captured on the modified electrode, and then ferrocene labeled polymyxin B (FcPMB) was captured on the LPS adsorbed electrode via the LPS‐PMB affinity interaction. The adsorbed FcPMB provided an amplified response with Fe2+ ions, and the current response was dependent on the amount of captured LPS (LOD=2.0 ng/mL). This was due to the efficient accumulation of the obtained current for LPS and the very low noise made possible by the ultraflat surface. |
| Keywords: | Nanocarbon film electrode Lipopolysaccharide Poly‐ε ‐lysine Electrochemical detection Signal amplification |