Evaluation of potential cationic probes for the detection of proline and betaine |
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| Authors: | Umme Kalsoom Michael C Breadmore Rosanne M Guijt Mary C Boyce |
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| Institution: | 1. Centre for Ecosystem Management, School of Natural Sciences, Edith Cowan University, Perth, Western Australia, Australia;2. ACROSS, School of Physical Sciences: Chemistry, University of Tasmania, Hobart, Tasmania, Australia;3. ACROSS, Pharmacy School of Medicine, University of Tasmania, Hobart, Tasmania, Australia |
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| Abstract: | Osmoregulants are the substances that help plants to tolerate environmental extremes such as salinity and drought. Proline and betaine are two of the most commonly studied osmoregulants. An indirect UV CE method has been developed for simultaneous determination of these osmoregulants. A variety of reported probes and compounds were examined as potential probes for the indirect detection of proline and betaine. Mobility and UV‐absorption properties highlighted sulfanilamide as a potential probe for indirect analysis of proline and betaine. Using 5 mM sulfanilamide at pH 2.2 with UV detection at 254 nm, proline and betaine were separated in less than 15 min. The LODs for proline and betaine were 11.6 and 28.3 μM, respectively. The developed method was successfully applied to quantification of these two osmoregulants in spinach and beetroot samples. |
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| Keywords: | Betaine Cationic probes Indirect detection Osmoregulants Proline |
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