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ECD of Tyrosine Phosphorylation in a Triple Quadrupole Mass Spectrometer with a Radio-Frequency-Free Electromagnetostatic Cell |
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| Authors: | Valery G Voinov Samuel E Bennett Joseph S Beckman Douglas F Barofsky |
| Institution: | 1. Department of Chemistry, Oregon State University, Corvallis, OR, 97333, USA 4. Pacific Institute of Bioorganic Chemistry, Vladivostok, Russia 2. Department of Environmental and Molecular Toxicology, Oregon State University, Corvallis, OR, 97333, USA 3. Department of Biochemistry and Biophysics, Oregon State University, Corvallis, OR, 97333, USA |
| Abstract: | A radio frequency-free electromagnetostatic (EMS) cell devised for electron-capture dissociation (ECD) of ions has been retrofitted into the collision-induced dissociation (CID) section of a triple quadrupole mass spectrometer to enable recording of ECD product-ion mass spectra and simultaneous recording of ECD-CID product-ion mass spectra. This modified instrument can be used to produce easily interpretable ECD and ECD-CID product-ion mass spectra of tyrosine-phosphorylated peptides that cover over 50% of their respective amino-acid sequences and readily identify their respective sites of phosphorylation. ECD fragmentation of doubly protonated, tyrosine-phosphorylated peptides, which was difficult to observe with FT-ICR instruments, occurs efficiently in the EMS cell. Figure ? |
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