| A series of simple curcumin-derived colorimetric and fluorescent probes for ratiometric-pH sensing and cell imaging |
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| 作者姓名： | Guangjie Song Di Jiang Lei Wang Xiangzhong Sun Hongtian Liu Yanqing Tian Meiwan Chen |
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| 作者单位： | 1. State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau;2. Department of Materials Science and Engineering, Southern University of Science and Technology;3. School of Physics and Technology, Wuhan University |
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| 基金项目： | supported by the National Natural Science Foundation of China (Nos. 51922111, 21774054, 21574061)； |
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| 摘 要： | Colorimetric and fluorescent probes have emerged as a potent tool for pH sensing due to easy operation and high sensitivity. However, most of the existing bimodal probes require complicated synthesis,which greatly limits their wide applications. Herein, a simple fluorescent dye（called BFCUR） featuring a D-π-A-π-D conjugated system was developed from the natural polyphenol curcumin（CUR）. BFCUR exhibited significant red-shift in UV absorption and fluorescence emission as pH increased because of th...
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| 收稿时间： | 18 March 2021 |
A series of simple curcumin-derived colorimetric and fluorescent probes for ratiometric-pH sensing and cell imaging |
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| Guangjie Song,Di Jiang,Lei Wang,Xiangzhong Sun,Hongtian Liu,Yanqing Tian,Meiwan Chen.A series of simple curcumin-derived colorimetric and fluorescent probes for ratiometric-pH sensing and cell imaging[J].Chinese Chemical Letters,2022,33(1):339-343. |
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| Institution: | 1. State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao, China;2. Department of Materials Science and Engineering, Southern University of Science and Technology, Shenzhen 518055, China;3. School of Physics and Technology, Wuhan University, Wuhan 430072, China |
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| Abstract: | Colorimetric and fluorescent probes have emerged as a potent tool for pH sensing due to easy operation and high sensitivity. However, most of the existing bimodal probes require complicated synthesis, which greatly limits their wide applications. Herein, a simple fluorescent dye (called BFCUR) featuring a D-π-A-π-D conjugated system was developed from the natural polyphenol curcumin (CUR). BFCUR exhibited significant red-shift in UV absorption and fluorescence emission as pH increased because of the deprotonation of the phenolic hydroxyl groups, which resulted in the enhanced intramolecular charge transfer (ICT). The ratiometric pH detection of BFCUR was achieved with remarkable accuracy by monitoring both the absorbance ratio A500/A650 and the fluorescence intensity ratio I622/I743 under various pH values. In addition, the clear color changes of BFCUR under different pH conditions were visible, which enabled BFCUR to be used in test strips for rapid, visual pH detection. Moreover, BFCUR exhibited low cytotoxicity, and was successfully applied for intracellular pH detection, where the fluorescence intensity was linearly related to pH value. This study highlighted the great potential of CUR-derived BFCUR as colorimetric and fluorescent probes for ratiometric-pH sensing and cell imaging. |
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