Silicon-substituted rhodamines for stimulated emission depletion fluorescence nanoscopy |
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| Authors: | Ning Wang Yumeng Hao Xiaowei Feng Haidan Zhu Dazhi Zhang Ting Wang Xiaoyan Cui |
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| Institution: | 1. Department of Chemistry, School of Chemistry and Molecular Engineering, East China Normal University, Shanghai 200241, China;2. Department of Organic Chemistry, College of Pharmacy, Naval Medical University (Second Military Medical University), Shanghai 200433, China |
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| Abstract: | As an essential part in the toolbox of super-resolution microscopy, stimulated emission depletion (STED) nanoscopy has been widely explored in revealing the substructure and bioactivities in fluorescence imaging. Among the applied STED fluorophores, silicon-substituted rhodamines (SiRs) belong to one of the most extensively employed fluorophores. The carboxy-SiR was favored in STED bioimaging with many advantages, including reliable photostability, cell permeability, tunable fluorogenicity, feasible structural decoration and so on. We reviewed the research of carboxy-SiR in the STED nanoscopy and hopefully this can inspire more efforts in the design and application of STED fluorophores. |
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