梯度加压毛细管电色谱同时分离大黄提取液中5种蒽醌类化合物

建立了同时分离药用大黄提取液中大黄酸、芦荟大黄素、大黄素、大黄酚和大黄素甲醚5种蒽醌类活性成分的梯度加压毛细管电色谱的新方法.实验结果显示,大黄提取液中的5种蒽醌化合物可在22min内完全分离,梯度洗脱微柱液相色谱的柱效为等度洗脱微柱液相色谱的6.63倍,梯度毛细管电色谱的柱效为梯度微柱液相色谱的4.6倍.

Simultaneous Separation of Five Anthraquinone Compounds in Chinese Herb Rhubarb Extracts by Pressurized Gradient Capillary Electrochromatography

A pressurized capillary electochromatography with gradient elution for simultaneous separation of five active anthraquinone compounds in Chinese herb rhubarb extracts has been proposed. The experimental results show that under the optimal conditions the five anthraquinone compounds of Rhein, Aloe-emodin, Chrysophanol, Emodin and Physcion in Chinese herb Rhubarb extracts can be completely separated in 22 min. The column efficiency obtained by capillary electrochromatography with gradient elution is 4.6 times that by μ-HPLC with gradient elution, and the latter is 6.63 times that by μ-HPLC with isocratic elution. The developed method hasthe advantages of higher separation capacity, faster separation rate and less consumption of samples and solvents compared with previously reported HPLC and CE methods, and thus may be applied to the separation and determination of active components in complex matrix systems.