A sensitive dual signal amplification method for western blotting based on antibody-functionalised graphene oxide and gold nanoparticles |
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| Authors: | Lin Hongjun Huo Jingrui Zhang Aihong Liu Yingfu Wang Qingming Cai Yun Ying Wantao Qin Weijie Zhang Yangjun Qian Xiaohong |
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| Affiliation: | State Key Laboratory of Proteomics, Beijing Proteome Research Center, Beijing Institute of Radiation Medicine, Beijing 102206, China. |
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| Abstract: | ![]()
In this study, we report an ultrasensitive western blotting method using antibody-functionalised graphene oxide sheets and gold nanoparticles. Additionally, the cost is reduced greatly by conjugating two different primary antibodies on gold nanoparticles (C. Welinder and L. Ekblad, J. Proteome Res., 2011, 10, 1416-1419). |
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