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土壤微生物ATP、脱氢酶和尿素酶活性的ED50值评价外源镧的生物毒性
引用本文:陈浮,杨桂山,曹慧,王腊春,彭补拙.土壤微生物ATP、脱氢酶和尿素酶活性的ED50值评价外源镧的生物毒性[J].无机化学学报,2002,18(8):827-832.
作者姓名:陈浮  杨桂山  曹慧  王腊春  彭补拙
作者单位:1. 南京大学城市与资源学系水土模拟实验室,南京,210093;中国科学院南京地理与湖泊研究所,南京,210009
2. 中国科学院南京地理与湖泊研究所,南京,210009
3. 中国科学院南京土壤研究所,南京,210009
4. 南京大学城市与资源学系水土模拟实验室,南京,210093
基金项目:国家自然科学基金重点项目(No.49831070),中国科学院知识创新工程项目(No.KZCX2-310、311),中国科学院红壤生态实验室开放基金联合资助(No.02008)
摘    要:0IntroductionRareearthsarenowappliedwidelyinChina,whichcanimprovecropsyieldsandthetheirqualit-ies犤1犦.Thebeneficialeffectsmaybeduetothestimu-latoryeffectsoftheseelementsonthenutrientuptakebyplantsorontheincreasingofchlorophyllsynthesisintheplants犤2犦.Whilealotofresearcheshavebeendoneontheimprovednutritionofcropsafterapplica-tionofrareearths,muchlessattentionhasbeenpaidtothedeteriorationofsoilqualityduetotheapplicationofrareearthsforyears犤3犦.Scientistshavediscoveredthataccumula…

关 键 词:外源镧  土壤微生物ATP  脱氢酶活性  尿素酶活性  ED50

The ED50 Values for Assessing Lanthanum Toxicity on Microbial ATP Content and Dehydrogenase and Urease Activities of Paddy Soil
CHEN Fu,YANG Gui-Shan,CAO Hui,WANG La-Chun and PENG Bu-Zhou.The ED50 Values for Assessing Lanthanum Toxicity on Microbial ATP Content and Dehydrogenase and Urease Activities of Paddy Soil[J].Chinese Journal of Inorganic Chemistry,2002,18(8):827-832.
Authors:CHEN Fu  YANG Gui-Shan  CAO Hui  WANG La-Chun and PENG Bu-Zhou
Institution:Laboratory of Soil and Water Simulating, Department of Urban and Resources Science, Nanjing University, Nanjing 210093;Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing 210009,Nanjing Institute of Geography and Limnology, Chinese Academy of Sciences, Nanjing 210009,Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210009,Laboratory of Soil and Water Simulating, Department of Urban and Resources Science, Nanjing University, Nanjing 210093 and Laboratory of Soil and Water Simulating, Department of Urban and Resources Science, Nanjing University, Nanjing 210093
Abstract:Rare earths are applied widely in Chinese agriculture to improve crops nutrition and incidentally in fertilizers, as yet little is known of their effect on the biological function of the soil. An incubation experiment was performed to assess lanthanum toxicity on microbial ATP content and dehydrogenase and urease activities in paddy soil, which was assayed after adding La for 4h, 1d, 1, 4, 8, and 16 weeks. The relative ED50 values were calculated by two kinetic models (model 1 and model 2) and one sigmoidal dose-response model (model 3). Model 1 was successful in calculating the ED50 values for the ATP content, dehydrogenase and urease activities. The ED50 values relating to ATP content and dehydrogenase and urease activities were 1086.5 and 897.8 and 1112.5 mg La kg-1 soil, re-spectively. The ED50 values of ATP content and dehydrogenase activity increase with incubation time, but the ED50 value of urease activity decreases. After 16 weeks, La amendment has no inhibition of urease activity. The present dosage of mixed rare earths, about 0.15mg kg-1 soil year-1, applied in agriculture can hardly affect ATP content and dehydrogenase and urease activities in the paddy soil even over a long period.
Keywords:exogenous lanthanum  soil microbial ATP  dehydrogenase activity  urease activity    ED50
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