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Eicosapentaenoic acid release from the red alga Pachymeniopsis lanceolata by enzymatic degradation |
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| Authors: | Ritsuko Matsukawa Kiyotaka Hatakeda Shota Ito Yukiyo Numata Hidetoshi Nakamachi Yasushi Hasebe Shunichi Uchiyama Masahiro Notoya Zvy Dubinsky Isao Karube |
| Institution: | (1) Research Center for Advanced Science and Technology, The University of Tokyo, 153-8904 Tokyo, Japan;(2) National Industrial Research Institute, Tohoku, 983-0036 Sendai-shi, Japan;(3) Department of Environmental Engineering, Saitama Institute of Technology, 369-0293 Saitama, Japan;(4) Laboratory of Applied Phycology, Tokyo University of Fisheries, 108-8477 Tokyo, Japan;(5) Department of Life Sciences, Bar-Ilan University, 52900 Ramat-Gan, Israel |
| Abstract: | Forty-eight species of seaweeds from Japanese waters were screened for the valuable polyunsaturated fatty acids eicosapentaenoic acid (EPA). The eight species that contained the highest levels of these compounds were analyzed in detail. Of all species tested the red alga Pachymeniopsis lanceolata contained the highest EPA concentration, and it was present as both the free and bound forms. EPA constituted 38.7% of total fatty acids, and polar lipids were the main constituent of the total lipids in P. lanceolata. EPA was obtained from the marine algae P. lanceolata by enzymatic hydrolysis of the total lipids extract using phospholipase A2(PLA2). The release of EPA reached a plateau after 10 min of enzymatic treatment. These results suggest that P. lanceolata is a useful natural source of EPA and that PLA2 treatment is a convenient method for obtaining EPA from the red alga. |
| Keywords: | Eicosapentaenoic acid phospholipase A2 enzymatic hydrolysis seaweeds Pachymeniopsis lanceolata |
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