注塑型聚甲基丙烯酸甲酯多通道微流控芯片的研制及其性能考察

微流控芯片技术因具有微量、快速、高效和高通量等特点，已成为分析化学领域中的研究热点之一．在微流控芯片中，最常见的可用作芯片的材料为玻璃、石英和各种塑料．玻璃和石英有很好的电渗性和光学性质，可采用标准的刻蚀工艺加工和用化学方法进行表面改性，但加工成本较高，封接难度较大．

Microfabrication and Performance of Poly(methyl methacrylate) Microfluidic Chip with Multi-channels

The microchip capillary electrophoresis devices were fabricated by using poly(methyl methacrylate)(PMMA) plastic material via an injection-molding process. The molded devices were enclosed by utilizing a mixed organic solvent to another PMMA film. The channel structure was very well defined and the molded channel surfaces were very smooth. The transmissivity was in the range from 91% to 93%(at the wavelength of 400—1 000 nm). In comparison to glass microchannels, the electroosmotic flow(EOF) in native PMMA channels was low. DNA marker separation was demonstrated in these PMMA devices with a high-resolution separation of double-stranded DNA fragments, chip-to-chip and the run-to-run reproducibility was good, and the relative standard deviation(%) values were below 2.2% for run-to-run data and 2.3% for the chip-to-chip comparisons. The PCR amplification products and proteins were analyzed on the PMMA chips. Such devices lead to the production of low-cost, disposable chips suitable for a variety of separation applications, including DNA sizing, DNA sequencing, protein and medical analysis. The detection limits of Rhodamine 6G dye for the unmodified PMMA chip and the modified PMMA chips were 1.0×10-10 and 6.67×10-13 mol/L, respectively.