荧光分光光度法测定中药对过氧化氢的清除率

基于苯胺被H2O2氧化为硝基苯使其荧光猝灭的原理建立了一种测定中药对H2O2清除率的新方法。研究表明：苯胺荧光在pH7～8之间有最大强度；乙醇浓度为50%（V/V）时，瑞利散射干扰最小，荧光强度最大；苯胺浓度为110mmol/L，H2O2的浓度小于1mmol/L时，苯胺荧光猝灭程度与H2O2浓度呈线性关系，测定了中药厚朴和杜仲对H2O2的清除率，结果与鲁米诺化学发光法测定值一致。

Determination of Eliminating Ratio of Chinese Traditional Medicines for Hydrogen Peroxide by Fluorescence Spectrophotometry

Based on principle that aniline can be oxidized as nitrobenzene and its fluorescence quenched by H2O2, a new method is developed to determine eliminating ratio of Chinese traditional medicines for H2O2 . It is shown that fluorescence of aniline is maximum when pH value is between 7 and 8. Rayleigh scattering interference is minimum and fluorescence is maximum when the concentration of ethanol in sample solution is 50%(V/V) . Linear relation between the quenching level of aniline fluorescence and the concentration of H2O2 is established when the concentration of 11202 is below 1 mmol/L. Eliminating ratios of Cortex Magnoliae officinalis and Cortex Eucommia for H2O2 are determined. The results are identical with those of luminol chemiluminescence.