荧光团杂化纳米SiO2微球作为生物标记探针的应用研究

近年来 ,无机发光量子点1,2 ] 、荧光纳米乳液微球3 ,4 ] 及发光团掺杂 Si O2 纳米粒子5] 等纳米荧光探针的出现 ,为生物标记提供了新的发展领域 .将有机染料以共价方式包埋在 Si O2 中所得的复合材料具有独特的光学性质 ,然而其在生物标记方面的应用并未得到重视6 ,7] .本实验通过控制荧光团修饰的硅烷前体在反相胶束体系中的水解缩合 ,合成了用于生物染色和诊断的高灵敏度、高稳定性的新型荧光团杂化纳米 Si O2 微球 ( NFHS微球 ) .在 NFHS微球中 ,荧光团以共价方式地均匀分散在 Si O2 网络结构中 ,避免了与外界体系中溶解氧的…

Nanometer Fluorescent Hybrid Silica Particle as Ultrasensitive and Photostable Biological Labels

Recent advances in the nanomaterials, such as luminescent quantum dots, latex fluorescent nanospheres and dye-doped silica nanoparticles, have opened a promising field toward the development of luminescent biolabel. In this paper, we develop a kind of novel nanometer-sized fluorescent hybrid silica(NFHS) particles used as a sensitive and photostable fluorescent probe in biological staining and diagnostics. The NFHS particles are prepared by controlled hydrolysis of the fluorophore silica precursor using the reverse micelle technique. The fluorophores are dispersed homogeneously in the silica network of the NFHS particles and well protected from the environmental oxygen. In comparison with single organic fluorophores without incorporation, these nanoparticle probes are brighter, more stable against photobleaching and do not suffer from intermittent on/off light emission(blinking). The NFHS particles have also shown unique advantages over the existing common organic fluorophores, quantum dots, and latex-based fluorescent particles for biomolecule recognition in the following four major points: easy preparation, good photostability, high sensitivity, and low toxicity. The approach proposed in this article for making NFHS nanoparticles is a general one, and it is not restricted to a particular type of fluorophore molecule as selected in this study.