|
|||||
|
|
| HPLC-MS/MS检测含蛋白质辐照食品中的邻酪氨酸 | |
| 引用本文： | 王敏,张秀芹,杨振宇,仲维科,陆地,郑文杰.HPLC-MS/MS检测含蛋白质辐照食品中的邻酪氨酸[J].分析测试学报,2011,30(10):1187-1190. |
| 作者姓名： | 王敏 张秀芹 杨振宇 仲维科 陆地 郑文杰 |
| 作者单位： | 1. 上海出入境检验检疫局,上海,200135 2. 中国检验检疫科学研究院,北京,100022 3. 烟台出入境检验检疫局,山东,烟台,264000 4. 天津出入境检验检疫局,天津,300457 |
| 基金项目： | 质检公益性行业科研专项项目（200910142） |
| 摘 要： | 应用液相色谱-串联质谱(HPLC- MS/MS )技术建立了含蛋白质辐照食品中邻酪氨酸的检测方法.样品经胰蛋白酶于37℃水解过夜,正己烷去脂,乙酸锌沉淀蛋白净化后,HPLC-MS/MS检测.方法的定量下限为0.1 mg/kg,邻酪氨酸在0.1,0.5,1.0 mg/kg加标水平的回收率为80%-97%,相对标准偏差(R... |
| 关 键 词： | 邻酪氨酸 含蛋白质辐照食品 液相色谱-串联质谱 |
Determination of o-Tyrosine in y-Ray Irradiated Protein-rich Food by HPLC-MS/MS |
|
| WANG Min,ZHANG Xiu-qin,YANG Zhen-yu,ZHONG Wei-ke,LU Di,ZHENG Wen-jie.Determination of o-Tyrosine in y-Ray Irradiated Protein-rich Food by HPLC-MS/MS[J].Journal of Instrumental Analysis,2011,30(10):1187-1190. | |
| Authors: | WANG Min ZHANG Xiu-qin YANG Zhen-yu ZHONG Wei-ke LU Di ZHENG Wen-jie |
| Institution: | WANG Min1,ZHANG Xiu-qin1,YANG Zhen-yu1,ZHONG Wei-ke2,LU Di3,ZHENG Wen-jie4 (1.Shanghai Entry-Exit Inspection and Quarantine Bureau,Shanghai 200135,China,2.Inspection and Quarantine Research Academy of China,Beijing 100022,3.Yantai Entry-Exit Inspection and Quarantine Bureau,Yantai 264000,4.Tianjin Entry-Exit Inspection and Quarantine Bureau,Tianjin 300457,China) |
| Abstract: | A liquid chromatography-tandem mass spectrometric method was developed for the determination of o-tyrosine in γ-ray irradiated protein-rich food.The sample was homogenized and hydrolyzed with parenzyme at 37 ℃ for 24 h.The digestion solution was purified with n-hexane eliminating fat,deproteinated with zinc acetic acid solution,and then determined by HPLC-MS/MS.The quantitation limit was 0.1 mg/kg.The recoveries of o-tyrosine at three spiked levels of 0.1,0.5 and 1.0 mg/kg were in the range of 80%-97%,with RSDs(n=6) of 6.3%-12.9%.The o-tyrosine contents in chicken,pork,beef,fish and shrimp irradiated with 2,5,10 kGy of γ-ray ranged from 0.095 mg/kg to 0.677 mg/kg,which were obviously higher than those in the unirradiated meat samples,and presented linear relationships with the irradiance dose.The method showed good reproducibility and sensitivity. |
| Keywords: | o-tyrosine γ-ray irradiated protein-rich food HPLC-MS/MS |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |
| 点击此处可从《分析测试学报》浏览原始摘要信息 | |
| 点击此处可从《分析测试学报》下载免费的PDF全文 | |