| 大黄素与DNA相互作用方式的研究 |
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| 引用本文： | 苏布道,廖立敏,雷光东,王碧.大黄素与DNA相互作用方式的研究[J].分析测试学报,2011,30(6):600-606. |
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| 作者姓名： | 苏布道 廖立敏 雷光东 王碧 |
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| 作者单位： | 1.内江师范学院化学化工学院,2.内江师范学院学科建设与研究生工作处 |
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| 基金项目： | 内江师范学院自然科学基金资助项目（09NJZ-5）；四川省教育厅青年基金资助项（09ZB036） |
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| 摘 要： | 通过分子荧光分析、紫外可见吸收光谱及粘度分析以及与溴化乙锭的竞争性实验对大黄素与DNA之问的作用模式进行了考察.在pH 7.4,低离子强度(30 mmol/L NaCl)及恒温37℃条件下反应35 min,大黄素的荧光强度随DNA加入浓度的增加而递减,紫外可见吸收光谱在380、475 nm处出现等吸收点并红移5nm,表...
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| 关 键 词： | 大黄素 DNA 嵌入式 荧光 紫外可见吸收光谱 溴化乙锭 猝灭 |
Study on Mechanism of the Interaction of Emodin and DNA |
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| SU Bu-dao,LIAO Li-min,LEI Guang-dong,WANG Bi.Study on Mechanism of the Interaction of Emodin and DNA[J].Journal of Instrumental Analysis,2011,30(6):600-606. |
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| Authors: | SU Bu-dao LIAO Li-min LEI Guang-dong WANG Bi |
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| Institution: | SU Bu-dao1,LIAO Li-min1,LEI Guang-dong2,WANG Bi1(1.College of Chemistry and Chemical Engineering,Neijiang Normal University,Neijiang 641112,China,2.The Subject Building and Graduate Work Department,China) |
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| Abstract: | The mode of interaction of emodin and DNA was studied through fluorescence analysis,UV-visible absorption spectra,viscosity measurement and competition experiments with ethidium bromide.When the optimal conditions were set as follows: pH 7.4,ionic strength of 30 mmol/L NaCl,temperature of 37 ℃ and reaction time of 35 min,the fluorescence intensity of emodin was decreased with the increase of DNA concentration.The isosbestic points(380 nm and 475 nm) of emodin was red shifted by 5 nm in the presence of DNA,indicating that the complex of emodin and ctDNA was formed.Meanwhile,the fluorescence spectra of adenine and DNA showed the similar fluorescence quenching to that of DNA,indicating that emodin was embedded in base pairs and the hydrogen bond between adenine and cytosine was damaged. |
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| Keywords: | emodin DNA intercalate fluorescence UV-Vis absorption spectrum ethidium bromide quenching |
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