草甘膦的邻硝基苯磺酰氯柱前衍生高效液相色谱分析

以邻硝基苯磺酰氯(NBSC)为衍生化试剂,建立了柱前衍生草甘膦的反相高效液相色谱紫外检测法,并对衍生化条件进行了优化.最佳衍生化条件为:衍生温度25℃,反应时间10 min,硼砂缓冲溶液浓度0.25 mol/L(pH 9.0),草甘膦与NBSC的摩尔比为1:5.HPLC分析条件为:采用Lichrospher C18柱,...

Determination of Glyphosate by High Performance Liquid Chromatography with o-Nitrobenzenesulfonyl Chloride as Derivatization Reagent

A derivatization method for the determination of glyphosate was developed by using o-nitrobenzenesulfonyl chloride as derivatization reagent.The optimized conditions of derivatization were as follows:reaction temperature:25 ℃,reaction time:10 min,buffer solution:0.25 mol/L borate buffer(pH 9.0),the molar ratio of glyphosate to o-nitrobenzenesulfonyl:1∶5.The optimal chromatographic conditions were as follows:Lichrospher C18 column(5 μm,4.6 mm×250 mm),detection wavelength:230 nm,flow rate:1.0 mL/min,mobile phase:methanol-pH 5.5 0.05 mol·L-1phosphate buffer(20∶80).Under the optimal conditions,the calibration curve for glyphosate was linear in the range of 0.05-1.0 g·L-1 with a correlation coefficient(r2)of 0.999 3.The limit of detection was 6.25 mg·L-1.The method was simple,rapid and reliable,and was suitable for the determination of glyphosate in general laboratory.