Dynamic range of fluorescence detection and base-calling accuracy in DNA sequencer based on single-photon counting. |
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| Authors: | Dmitri N Gavrilov Boris Gorbovitski Mikhail Gouzman Georgiy Gudkov Andrei Stepoukhovitch Victor Ruskovoloshin Andrei Tsuprik Georgiy Tyshko Olga Bilenko Olga Kosobokova Serge Luryi Vera Gorfinkel |
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| Institution: | Department of Electrical and Computer Engineering, State University of New York, Stony Brook 11794, USA. gavrilov@ece.sunysb.edu |
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| Abstract: | Recently, we developed a family of high-performance automated capillary DNA sequencing instruments based on a single-photon detection of fluorescently labeled DNA fragments. Our machines employ digital and broadband techniques, essential for achieving superior instrument sensitivity and dynamic range. In the present paper, we discuss limitations of the instrument's performance caused by the nonlinearity of single-photon detectors as well as methods for nonlinearity compensation which increase the detection dynamic range and base-calling accuracy. |
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