GPC,CD and sedimentation analysis of poly-Lys and branched chain poly-Lys-poly-DL-Ala polypeptides |
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| Authors: | F Hudecz P Kovács S Kutassi-Kovács J Kajtár |
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| Institution: | (1) Research Group for Peptide Chemistry, Hungarian Academy of Sciences, Budapest;(2) Present address: Department of Colloid Science, L. Eötvös University, Budapest;(3) Institute of Organic Chemistry, L. Eötvös University, Budapest |
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| Abstract: | Three different poly-L-lysine (pLys) samples and a branched polypeptide poly-L-Lys-(DL-Ala)m] (poly-L-Lys-poly-DL-Ala, AK) were synthetized. Relative molar mass distribution, its average and the degree of polymerisation were determined by sedimentation analysis and gel permeation chromatography (GPC). The conformation of the polymers in solution was studied by circular dichroism (CD) spectroscopy at various pH values and ionic strengths. The data obtained by different methods were compared.A preliminary account of this work was read at the 4th Conference on Colloid Chemistry, Eger, 1983. |
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| Keywords: | Poly-L-Lys branched polypeptides poly-L-Lys-poly-DL-Ala GPC of synthetic polypeptides sedimentation analysis |
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