HPLC enantioseparation of β2‐homoamino acids using crown ether‐based chiral stationary phase |
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| Authors: | Róbert Berkecz István Ilisz Aleksandra Misicka Dagmara Tymecka Ferenc Fülöp Hee Jung Choi Myung Ho Hyun Antal Péter |
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| Affiliation: | 1. Department of Inorganic and Analytical Chemistry, University of Szeged, Szeged, Hungary. Fax: +36‐62‐420505;2. Department of Chemistry, University of Warsaw, Warsaw, Poland;3. Medical Research Center, Polish Academy of Sciences, Warsaw, Poland;4. Institute of Pharmaceutical Chemistry, University of Szeged, Szeged, Hungary;5. Department of Chemistry and Chemistry Institute for Functional Materials, Pusan National University, Busan, South Korea |
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| Abstract: | RP high‐performance liquid chromatographic methods were developed for the enantioseparation of eleven unusual β2‐homoamino acids. The underivatized analytes were separated on a chiral stationary phase containing (+)‐(18‐crown‐6)‐2,3,11,12‐tetracarboxylic acid as chiral selector. The effects of organic (alcoholic) and acidic modifiers, the mobile phase composition and temperature on the separation were investigated. The structures of the substituents in the α‐position of the analytes substantially influenced the retention and resolution. The elution sequence was determined in some cases: the S enantiomers eluted before the R enantiomers. |
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| Keywords: | Column liquid chromatography β 2‐Homoamino acids (+)‐(18‐Crown‐6)‐2,3,11,12‐tetracarboxylic acid‐based chiral stationary phase |
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