Determination of Total Polyphenol Glycosides in Polygoni Cuspidati Rhizoma and Rumecis Radix |
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| Authors: | Hsiu‐Mei Chiang Shang‐Yuan Tsai Pei‐Ling Hsiao Chen‐Yuan Chiu Su‐Lan Hsiu Kuo‐Ching Wen |
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| Institution: | 1. Department of Cosmeceutics, China Medical University, Taichung, Taiwan, R.O.C.;2. School of Pharmacy, China Medical University, Taichung, Taiwan, R.O.C.;3. Institute of Pharmaceutical Chemistry, China Medical University, Taichung, Taiwan, R.O.C. |
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| Abstract: | Polyphenol glycosides are reported to be hydrolyzed to lower polar aglycones and then become absorbable in the intestine. It would be more reasonable if the total glycosides of active ingredients could be assayed. In this study, the contents of polyphenol glycosides in Chinese herbs—Polygoni cuspidati rhizoma and Rumecis radix—were studied. The contents of aglycones were determined in decoctions of the herbs before and after hydrolysis. The aglycones assayed were emodin and resveratrol for Polygoni cuspidati rhizoma, and emodin and chrysophanol for Rumecis radix. The results showed that the contents of aglycones in Rumecis radix and Polygoni cuspidati rhizoma increased by 9227% and 4930%, respectively, after hydrolysis. In addition, the content of emodin in Polygoni cuspidati rhizoma increased after hydrolysis (9187%), whereas the amount of resveratrol was decreased (40%) as heating time increased. The method in this study can be applied to the determinations of the contents of aglycones and their glycosides in these Chinese herbs for quality control. |
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| Keywords: | Chinese herbs Anthraquinone glycosides Polyphenols HPLC |
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