Simultaneous determination of pyrethroids from pesticide residues in porcine muscle and pasteurized milk using GC |
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Authors: | Sathya Khay A M Abd El‐Aty Jeong‐Heui Choi Eun‐Ho Shin Ho‐Chul Shin Jin‐Suk Kim Byung‐Joon Chang Chi‐Ho Lee Sung‐Chul Shin Ji Yoon Jeong Jae‐Han Shim |
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Institution: | 1. Natural Products Chemistry Laboratory, College of Agriculture and Life Science, Chonnam National University, Gwangju, Republic of Korea. Fax: +82‐62‐530‐0219;2. Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University, Kwangjin‐gu, Seoul, Republic of Korea;3. Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, Egypt;4. Department of Anatomy, College of Veterinary Medicine, Konkuk University, Kwangjin‐gu, Seoul, Republic of Korea;5. Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Kwangjin‐gu, Seoul, Republic of Korea;6. Department of Chemistry, Gyeonsang National University, Jinju, Republic of Korea;7. Residue and Chemicals Team, Korea Food and Drug Administration, Eunpyeong‐gu, Seoul, Republic of Korea |
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Abstract: | The principal goal of this work was to develop an efficient method for the simultaneous determination of four pyrethroid (PYR) insecticides, cyfluthrin, cyhalothrin, cypermethrin, and deltamethrin, in porcine muscle and pasteurized milk using liquid–liquid extraction (LLE). Sample extraction was carried out with and without additional column cleanup procedures, and the final determination was made using GC with electron‐capture detector (ECD). The pesticide identity was confirmed using GC‐MS in the SIM mode. Since there were minor differences between the extraction procedures, extraction without the additional cleanup procedure was used throughout the work. The method was validated by fortifying blank samples with half, two, and four times the maximum residue limit (MRL) of each PYR. The average recoveries (n = 6) ranged from 83.5 to 99.2% and 82.9 to 109% in porcine muscle and pasteurized milk, respectively. The repeatability of measurements expressed as RSDs, was in the range of 1.7–11.9 and 1.5–10.3% in porcine muscle and pasteurized milk, respectively. The LODs ranged from 3.3 to 9 and 3 to 8.1 ppm, whereas the LOQs ranged from 10 to 27.4 and 9 to 24.6 ppm, in porcine muscle and pasteurized milk, respectively. The applicability of the method was demonstrated by analyzing real samples collected from major cities in the Republic of Korea. No residues of the selected pesticides were detected in any of the samples. |
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Keywords: | Animal tissues GC Multiresidue Pyrethroids Simultaneous |
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