Single-molecule visualization of mRNA circularization during translation |
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| Authors: | Byungju Kim Jincheol Seol Yoon Ki Kim Jong-Bong Lee |
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| Affiliation: | 1.Department of Physics, Pohang University of Science & Technology (POSTECH), Pohang, 37673 Republic of Korea ;2.School of Interdisciplinary Bioscience and Bioengineering, POSTECH, Pohang, 37673 Republic of Korea ;3.Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon, 34141 Republic of Korea |
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| Abstract: | ![]()
Translation is mediated by precisely orchestrated sequential interactions among translation initiation components, mRNA, and ribosomes. Biochemical, structural, and genetic techniques have revealed the fundamental mechanism that determines what occurs and when, where and in what order. Most mRNAs are circularized via the eIF4E–eIF4G–PABP interaction, which stabilizes mRNAs and enhances translation by recycling ribosomes. However, studies using single-molecule fluorescence imaging have allowed for the visualization of complex data that opposes the traditional “functional circularization” theory. Here, we briefly introduce single-molecule techniques applied to studies on mRNA circularization and describe the results of in vitro and live-cell imaging. Finally, we discuss relevant insights and questions gained from single-molecule research related to translation.Subject terms: Single-molecule biophysics, Ribosome |
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