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A Schiff base fluorescence probe for highly selective turn-on recognition of Zn2+
Authors:Jinli Zhu  Yuhuan Zhang  Yihan Chen  Tongming Sun  Yanfeng Tang  Yang Huang  Qingqing Yang  Danyang Ma  Yipu Wang  Miao Wang
Institution:1. School of Chemistry and Chemical Engineering, Nantong University, Nantong 226019, PR China;2. School of Textiles, Nantong University, Nantong 226019, PR China
Abstract:A simple Schiff base CTS, synthesized between 2-hydroxy-1-naphthaldehyde and 2-benzylthio-ethanamine, was found to be a good turn-on fluorescence probe for the detection of Zn2+, due to the restriction of the rotation of the bond between C/>N and naphthalene ring and/or the blocking of the photo-induced electron transfer (PET) mechanism of the nitrogen atom to naphthalene ring. Excellent selectivity for Zn<sup>2+</sup> was evidenced, over many other competing ions, including Fe<sup>3+</sup>, Cr<sup>3+</sup>, Ni<sup>2+</sup>, Co<sup>2+</sup>, Fe<sup>2+</sup>,Mn<sup>2+</sup>, Ca<sup>2+</sup>, Hg<sup>2+</sup>, Pb<sup>2+</sup>, Cu<sup>2+</sup>, Mg<sup>2+</sup>, Ba<sup>2+</sup>, Cd<sup>2+</sup>, Ag<sup>+</sup>, Li<sup>+</sup>, K<sup>+</sup>, and Na<sup>+</sup>, in EtOH/HEPES buffer (95:5, v/v, pH<!-- --> <!-- -->=<!-- --> <!-- -->7.4). It was noteworthy that Cd<sup>2+</sup> had no interference with Zn<sup>2+</sup>. The stoichiometric complex of <strong>CTS</strong>-Zn<sup>2+</sup> was determined to be 2:1 for <strong>CTS</strong> and Zn<sup>2+</sup> in molar, based on the Job plot and single crystal X-ray diffraction data. The binding constant of the complex was 85.7<!-- --> <!-- -->M<sup>?2</sup> with a detection limit of 5.03<!-- --> <!-- -->×<!-- --> <!-- -->10<sup>?7</sup> <!-- -->M. The fluorescence bio-imaging capability of <strong>CTS</strong> to detect Zn<sup>2+</sup> in live cells was also studied. These results indicated that <strong>CTS</strong> could serve as a favorable probe for Zn<sup>2+</sup>.</td>
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Keywords:Schiff base  Turn-on fluorescence  Live cells
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