Study of the alkylation behaviors of antitumor agents to DNA using a quartz crystal resonator in PEG-DNA solution |
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Authors: | Zhao-Hui Lin Guo-Li Shen Ye Lin Ru-Qin Yu |
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Institution: | (1) Institute of Chemometrics and Chemical Sensing Technology, Human University, Changsha, 410082, P.R. of China, CN |
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Abstract: | The alkylation of DNA by antitumor agents such as mechlorethamine hydrochloride (mustargen), thiophosphoramide (TSPA), mitomycin
c (MMC), bleomycin-A5 and dacarbazine (DTIC) can be detected with quartz crystal resonators (QCR). In the course of alkylation, the resonator frequency
change in polyethylene glycol (PEG)-DNA solutions follows the cross-linking of DNA and the cleavage of the sugarphosphate
backbone of DNA. It is at least partly attributed to the viscosity change of the PEG-DNA solution and possibly to some extent
to the change of mass adsorbed on the QCR surface due to cross-linking and cleavage. Experimental results are consistent with
that expected from theory.
Received: 15 April 1996 / Revised: 3 July 1996 / Accepted: 9 July 1996 |
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