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Specific Adhesion of 3T3‐L1 Fibroblast onto Uridine‐Containing Polystyrene
Authors:Kenichi Hatanaka  Junji Oishi  Akinori Tsuda  Sae Matsunaga  Megumi Kunou  Yoshihide Yachi  Maria Carmelita Kasuya  Tatsuyuki Okinaga
Abstract:A hydrophobic 96‐well multiplate was incubated with aqueous poly(uridine 5′‐p‐styrenesulfonate) (PUSS). Analysis of the PUSS‐coated surface indicated that the surface incubated at higher polymer concentration (50 mg·mL–1) was hydrophilic while the surface incubated at lower polymer concentration (1 mg·mL–1) was hydrophobic. Adhesion of 3T3‐L1, which has GalTase on the cell membrane, on the hydrophobic PUSS‐coated surface was greater than on the non‐coated surface. 3T3‐L1 adhesion on the hydrophilic PUSS‐coated surface was even greater. On the other hand, the adhesion of HeLa cells, which did not show GalTase on the cell membrane, on the hydrophilic surface was quite weak. It may well be said that the 3T3‐L1 adhesion on the hydrophilic PUSS‐coated surface was GalTase‐mediated.
Keywords:adhesion  biological application of polymers  enzymes  molecular recognition
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