导数同步荧光法同时测定1-萘胺和2-萘胺

为同时测定2种萘胺异构体的含量，研究了1-萘胺和2-萘胺的同步荧光光谱及其一阶导数同步荧光光谱，利用零交点法避免了它们之间的干扰。在pH=7.5的KH2PO4-NaOH缓冲溶液中，波长差为120 nm的条件下，测定了1-萘胺和2-萘胺的同步荧光。进一步对2种萘胺的同步光谱做一阶导数处理，分别在1-萘胺和2-萘胺的导数同步荧光光谱为零的259和290 nm处读取另一种异构体的信号值。该值与浓度呈线性关系，线性范围均在4.0×10-7～2.0×10-5 mol/L；1-萘胺和2-萘胺的检出限分别是4.0×10-8和2.9×10-8 mol/L；RSD均在5%以下。该方法用于产品中2种萘胺的同时测定，获得满意结果。

Simultaneous Determination of 1-Naphylamine and 2-Naphylamine by Derivative Synchronous Fluorescent Method

A fluorescent method for the synchronous determination of 1-naphylamine and 2-naphylamine was developed. In order to avoid the interference from the two isomers, the synchronous fluorescence and first-order-derivative synchronous fluorescence spectra of the two isomers of naphylamine were studied, and the zero-crossing method for reading spectral intensities was adopted. At pH=7.8(phosphate buffered water solution) and Δλ=120 nm, the synchronous fluorescence peaks were at 323 nm and 290 nm for 1-napylamine and 2-naphylamine, respectively. The first order derivative synchronous fluorescence intensities varied linearly with 1-naphylamine and 2-naphylamine concentrations in a range of 4.0×10-7~2.0×10-5 mol/L. The relative standard deviations(RSDs) of them were all below 5%. The limits of detection were 4.0×10-8 and 2.9×10-8 mol/L for 1-naphylamine and 2-naphylamine, respectively. The method was applied to the simultaneous determination of the two naphylamine isomers with satisfactory results.