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Planar thiol-sensitive sensor elements for the determination of butyrylcholinesterase activity and analysis of its inhibitors |
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| Authors: | A. V. Eremenko T. A. Prokopkina V. E. Kasatkin T. A. Osipova I. N. Kurochkin |
| Affiliation: | 1. Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, Moscow, Russia 2. Department of Chemistry, Moscow State University, Moscow, 119991, Russia 3. Frumkin Institute of Physical Chemistry and Electrochemistry, Russian Academy of Sciences, Moscow, Russia |
| Abstract: | A method for the analysis of the thiocholine concentration, as well as the activity of the butyrylcholinesterase (BChE) enzyme and its inhibitors, using thiol-sensitive planar sensors has been proposed. Thiocholine sensitivity of the sensors was achieved by a mediator layer of manganese dioxide formed on a working carbon electrode. The detection limits for BChE and its inhibitor diazinon were 1 × 10?10 M and 1 × 10?9 M, respectively. The approach that was developed simplifies the analysis of inhibitors. It could be used for water quality and environmental monitoring. |
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