Evidence for neuroprotective properties of human umbilical cord blood cells after neuronal hypoxia <Emphasis Type="Italic">in vitro</Emphasis> |
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| Authors: | Susann Hau Doreen M Reich Markus Scholz Wilfried Naumann Frank Emmrich Manja Kamprad Johannes Boltze |
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| Institution: | (1) Fraunhofer-Institute for Cell Therapy and Immunology, Deutscher Platz 5e, 04103 Leipzig, Germany;(2) University of Leipzig, Institute of Clinical Immunology and Transfusion Medicine, Johannisallee 30, 04103 Leipzig, Germany;(3) Faculty of Biology, Pharmacy and Psychology, University of Leipzig, Institute of Zoology II, Talstrasse 33, 04103 Leipzig, Germany;(4) University of Leipzig, Institute of Medical Informatics, Statistics and Epidemiology, Haertelstrasse 16-18, 04107 Leipzig, Germany;(5) Translational Centre for Regenerative Medicine, Philipp-Rosenthal-Strasse 55, 04103 Leipzig, Germany |
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| Abstract: | Background One of the most promising options for treatment of stroke using adult stem cells are human umbilical cord blood (HUCB) cells
that were already approved for therapeutic efficacy in vivo. However, complexity of animal models has thus far limited the understanding of beneficial cellular mechanisms. To address
the influence of HUCB cells on neuronal tissue after stroke we established and employed a human in vitro model of neuronal hypoxia using fully differentiated vulnerable SH-SY5Y cells. These cells were incubated under an oxygen-reduced
atmosphere (O2< 1%) for 48 hours. Subsequently, HUCB mononuclear cells (MNC) were added to post-hypoxic neuronal cultures. These cultures
were characterized regarding to the development of apoptosis and necrosis over three days. Based on this we investigated the
therapeutic influence of HUCB MNC on the progression of apoptotic cell death. The impact of HUCB cells and hypoxia on secretion
of neuroprotective and inflammatory cytokines, chemokines and expression of adhesion molecules was proved. |
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