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| 反相胶束介质中漆酶催化反应中间体信息的捕捉及其解析 | |
| 引用本文： | 刘志洪,沈萍,蔡汝秀. 反相胶束介质中漆酶催化反应中间体信息的捕捉及其解析[J]. 武汉大学学报(理学版), 2003, 49(2): 149-152 |
| 作者姓名： | 刘志洪 沈萍 蔡汝秀 |
| 作者单位： | 1. 武汉大学,化学与分子科学学院,湖北,武汉,430072;武汉大学,生命科学学院,湖北,武汉,430072 2. 武汉大学,生命科学学院,湖北,武汉,430072 3. 武汉大学,化学与分子科学学院,湖北,武汉,430072 |
| 基金项目： | 国家自然科学基金资助项目 ( 2 9775 0 5 1) |
| 摘 要： | 利用反相胶束独特的介质特性，捕捉到漆酶催化反应的中间体光谱动力学信号，实现了对酶催化反应过程的在线光谱研究．通过解析反应过程中在线测得的动力学光谱数据矩阵，解得中间体的生成和衰减均为一级反应，并求出实验条件下的反应速率常数．通过实验数据与解析结果的比较证实了方法的合理性． |
| 关 键 词： | 反相胶束介质 漆酶 酶催化反应 中间体 光谱动力学信号 反应速率 反应级数 |
| 文章编号： | 1671-8836(2003)02-0149-04 |
| 修稿时间： | 2002-11-10 |
Study on the Capture of Spectral Information of Laccase-Catalyzed Intermediate in Reversed Micelles and Its Resolution |
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| LIU Zhi-hong ,,SHEN Ping ,CAI Ru-xiu. Study on the Capture of Spectral Information of Laccase-Catalyzed Intermediate in Reversed Micelles and Its Resolution[J]. JOurnal of Wuhan University:Natural Science Edition, 2003, 49(2): 149-152 | |
| Authors: | LIU Zhi-hong SHEN Ping CAI Ru-xiu |
| Affiliation: | LIU Zhi-hong 1,2,SHEN Ping 2,CAI Ru-xiu 1 |
| Abstract: | The kinetic spectrum of the intermediate of laccase-catalyzed reaction was captured by making use of unique properties of reversed micelles. Hence the kinetics of the enzymatic reaction was studied on-line. Through the resolution of the experimental data matrix, the formation and decay of the intermediate was found to be first-order reactions. The rate constants of the two steps of reaction were obtained. Calculated results were compared with experimental data, which showed the reliability of the resolution as well as the limitation of some traditional methods on dealing with such a on-line-analysis problem. |
| Keywords: | laccase intermediate reverse micelle |
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