| Novel Rearrangement of Small Peptides in Electrospray Ionization Tandem Mass Spectrometry |
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| 作者姓名： | 陈益 王津 刘晓红 孙命 陈晶 汪洋 赵玉芬 |
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| 作者单位： | [1]KeyLaboratoryofChemicalBiology,DepartmentofChemistry,ZhengzhouUniversity,Zhengzhou,Henan450052,China [2]CepartmentofChemistry,PekingUniversity,Beijing100871,China [3]KeyLaboratoryforBioorganicPhosphorusChemistry,MinistryofEducation,DepartmentofChemistry,SchoolofLifeSciencesandEngineering,TsinghuaUniversity,Beijing100084,China |
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| 基金项目： | Project supported by the Chinese National Natural Science Foundation (No. 20175026)，the Ministry of Science and Technology，the visiting scholar fund of the Chinese Education Ministry，and Tsinghua University. |
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| 摘 要： | Electrospray ionization mass spectrometry (ESI-MS) is a powerful method for sequencing peptides. A novel fragmentation pattern with the loss of a neutral fragment of 45 Da was observed with the dipeptides, tripeptides,tetrapeptides and pentapeptides containing phenylalanine or histidine residues. A novel rearrangement reaction with the extrusion of a formamide piece was studied and the rearrangement mechanism was proposed and confirmed by deuterium labeling experiments with ESI-MS^n and high-resolution mass spectrometry. These findings are potentially helpful in identifying the specific sequence pattern in the peptide sequencing.
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| 关 键 词： | 电喷雾串联质谱 重整反应 小肽 二肽 三肽 四肽 五肽 苯丙氨酸 组氨酸 甲酰胺 |
Novel Rearrangement of Small Peptides in Electrospray Ionization Tandem Mass Spectrometry |
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| CHEN,Yia,b WANG,Jinc LIU,Xiao-Hongb SUN,Mingb CHEN,Jinga JIANG,Yangb ZHAO,Yu-Fen,a,b a Key Laboratory of Chemical Biology.Novel Rearrangement of Small Peptides in Electrospray Ionization Tandem Mass Spectrometry[J].Chinese Journal of Chemistry,2004,22(5):477-481. |
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| Authors: | CHEN Yia b WANG Jinc LIU Xiao-Hongb SUN Mingb CHEN Jinga JIANG Yangb ZHAO Yu-Fen a b a Key Laboratory of Chemical Biology |
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| Institution: | CHEN,Yia,b WANG,Jinc LIU,Xiao-Hongb SUN,Mingb) CHEN,Jinga JIANG,Yangb ZHAO,Yu-Fen*,a,b) a Key Laboratory of Chemical Biology,Department of Chemistry,Zhengzhou University,Zhengzhou,Henan 450052,China b Key Laboratory for Bioorganic Phosphorus Chemistry,Ministry of Education,Department of Chemistry,School of Life Sciences and Engineering,Tsinghua University,Beijing 100084,China c Department of Chemistry,Peking University,Beijing 100871,China |
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| Abstract: | Electrospray ionization mass spectrometry (ESI-MS) is a powerful method for sequencing peptides. A novel fragmentation pattern with the loss of a neutral fragment of 45 Da was observed with the dipeptides, tripeptides, tetrapeptides and pentapeptides containing phenylalanine or histidine residues. A novel rearrangement reaction with the extrusion of a formamide piece was studied and the rearrangement mechanism was proposed and confirmed by deuterium labeling experiments with ESI-MSn and high-resolution mass spectrometry. These findings are poten-tially helpful in identifying the specific sequence pattern in the peptide sequencing. |
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| Keywords: | rearrangement small peptides electrospray ionization tandem mass spectrometry |
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