Choosing an equivalent replacement column for a reversed-phase liquid chromatographic assay procedure |
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| Authors: | Dolan J W Maule A Bingley D Wrisley L Chan C C Angod M Lunte C Krisko R Winston J M Homeier B A McCalley D V Snyder L R |
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| Affiliation: | BASi Northwest Laboratory, McMinnville, OR 97128, USA. |
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| Abstract: | The column selectivity parameters (H, S*, A, B and C) described in the preceding paper [L.R. Snyder, A. Maule, A. Heebsch, R. Cuellar, S. Paulson, J. Carrano, L. Wrisley C.C. Chan, N. Pearson, J.W. Dolan, J.J. Gilroy, J. Chromatogr. A 1057 (2004) 49-57] can be used to compare columns in terms of selectivity. A detailed procedure for such column comparisons is presented here, and evaluated by its use in finding suitable replacement columns for 12 different routine separations performed in five different pharmaceutical analysis laboratories. |
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