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Dynamic Hydrogen-Bonded Zinc Adeninate Framework (ZAF) for Immobilization of Catalytic DNA |
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| Authors: | Walaa S Baslyman Dr Othman Alahmed Dr Santanu Chand Somayah Qutub Prof Niveen M Khashab |
| Institution: | 1. Smart Hybrid Materials (SHMs) Laboratory, Advanced Membranes and Porous Materials Center, Physical Science and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal, 23955-6900 Saudi Arabia;2. Smart Hybrid Materials (SHMs) Laboratory, Advanced Membranes and Porous Materials Center, Physical Science and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal, 23955-6900 Saudi Arabia
Developmental Medicine Department, King Abdullah International Medical Research Center, King Saud Bin Abdulaziz University for Health Sciences, Ministry of National Guard-Health Affairs (MNG-HA), Riyadh, 11481 Saudi Arabia;3. Smart Hybrid Materials (SHMs) Laboratory, Advanced Membranes and Porous Materials Center, Physical Science and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal, 23955-6900 Saudi Arabia Department of Applied Chemistry, Graduate School of Engineering, The University of Tokyo, Bunkyo-ku, Tokyo, 113-8656 Japan |
| Abstract: | Effective immobilization and delivery of genetic materials is at the forefront of biological and medical research directed toward tackling scientific challenges such as gene therapy and cancer treatment. Herein we present a biologically inspired hydrogen-bonded zinc adeninate framework (ZAF) consisting of zinc adeninate macrocycles that self-assemble into a 3D framework through adenine-adenine interactions. ZAF can efficiently immobilize DNAzyme with full protection against enzyme degradation and physiological conditions until it is successfully delivered into the nucleus. As compared to zeolitic imidazolate frameworks (ZIFs), ZAFs are twofold more biocompatible with a significant loading efficiency of 96 %. Overall, our design paves the way for expanding functional hydrogen-bonding-based systems as potential platforms for the loading and delivery of biologics. |
| Keywords: | Gene Delivery Hydrogen-Bonded Organic Frameworks Immobilization Self-Assembly Zeolitic Framework |