Anti-Proliferative Effect of Allium senescens L. Extract in Human T-Cell Acute Lymphocytic Leukemia Cells |
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| Authors: | Jiyeon Kim Dae Han Lee Bazarragchaa Badamtsetseg Sangwoo Lee Soon Ae Kim |
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| Institution: | 1.Department of Medical Laboratory Science, College of Health Science, Dankook University, Cheonan 31116, Korea;2.Department of Pharmacology, School of Medicine, Eulji University, Daejeon 34824, Korea;3.Department of Environment and Forest Resources, Chungnam National University, Daejeon 34134, Korea;4.International Biological Material Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Korea; |
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| Abstract: | Allium species are well known plants distributed throughout the world, and they contain various bioactive components with different biological activities including anti-cancer effects. In this study, we investigated the inhibitory effect of Allium senescens L. (A.S.) extract on cell survival and IL-2-mediated inflammation in human T cell acute lymphocytic leukemia (T-ALL) Jurkat cells. Our results showed that A.S. extract induced caspase-dependent apoptosis of Jurkat cells with no significant cytotoxicity in the normal peripheral blood mononuclear cells. A.S. extract induced ROS generation through the activation of MAPK p38 phosphorylation. It also inhibited IL-2 mRNA expression and NF-κB signaling mediated by phorbol 12-myristate 13-acetate, and phytohemagglutinin. Combined treatment with A.S. extract and axitinib/dovitinib exerted enhanced inhibitory effects on T-ALL cell growth and IL-2 production. These results provide novel information on the potential use of A.S. extract as a therapeutic herbal agent for the treatment and prevention of T-ALL. |
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| Keywords: | Allium senescens L apoptosis axitinib dovitinib leukemia T-ALL |
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