| 荧光素单磷酸酯的合成和其应用于荧光光度法测定酸性磷酸酯酶 |
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| 引用本文： | 王流芳 吴安心. 荧光素单磷酸酯的合成和其应用于荧光光度法测定酸性磷酸酯酶[J]. 发光学报, 1993, 14(4): 372-377 |
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| 作者姓名： | 王流芳 吴安心 |
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| 作者单位： | 1. 兰州大学化学系, 兰州 730000;2. 兰州医学院药学系, 兰州 730000 |
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| 基金项目： | 国家及甘肃省自然科学基金 |
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| 摘 要： | 本文新合成了荧光素单磷酸酯,通过元素分析、红外、热谱确定了其结构,探讨了其生成机理,并将其应用到酸性磷酸酯的荧光分析,深入研究了它与酸性磷酸酯酶之间反应的条件、检测此酶的线性范围、检测限和米氏常效.
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| 关 键 词： | 荧光素单磷酸酯 酶的测定 荧光分析法 |
| 收稿时间： | 1992-11-10 |
SYNTHESIS OF FLUORESCEIN MONOPHOSPHATE AND ITS FLUOROMETRIC ANALYSIS OF ACIDIC PHOSPHATASE |
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| Wang Liufang Gong Guoquan Li Qingyu Tang Huian Hou Zijie. SYNTHESIS OF FLUORESCEIN MONOPHOSPHATE AND ITS FLUOROMETRIC ANALYSIS OF ACIDIC PHOSPHATASE[J]. Chinese Journal of Luminescence, 1993, 14(4): 372-377 |
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| Authors: | Wang Liufang Gong Guoquan Li Qingyu Tang Huian Hou Zijie |
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| Affiliation: | 1. Department of Chemistry, Lanzhou University, Lanzhou 730000;2. Lanzhou Medical College, Lanzhou 730000 |
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| Abstract: | Fluorescein monopyhosphate was synthesized with fluorescein and phosphorus oxychloride. The monophosphate was applied to the fluoro-metric analysis of acidic phosphatase. The reaction conditions of phos-phatase with fluorescein monophosphate, linear range for determination of the phosphatase, detection limitation and Michaelis constant were studied. |
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| Keywords: | fluorescein monophosphate acidic phosphatase fluoro-metric method |
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