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| 基于Sol-gel膜和多壁碳纳米管/铂纳米颗粒增效的电流型L-乳酸生物传感器 | |
| 引用本文： | 贺晓蕊,于华,葛慎光,张秀明,林青,朱晗,封烁,袁靓,黄加栋.基于Sol-gel膜和多壁碳纳米管/铂纳米颗粒增效的电流型L-乳酸生物传感器[J].分析化学,2010,38(1). |
| 作者姓名： | 贺晓蕊 于华 葛慎光 张秀明 林青 朱晗 封烁 袁靓 黄加栋 |
| 作者单位： | 1. 济南大学化学化工学院,济南,250022 2. 泉城学院,济南,250022 3. 医学与生命科学学院,济南,250022 |
| 基金项目： | 国家自然科学基金(Nos.30700535,30972056);;国家863计划(No.2007AA10Z430);;中韩(NSFC-NRF)联合资助合作与交流项目(No.30911140278)资助 |
| 摘 要： | 构建了基于多壁碳纳米管(Multi-walled carbon nanotubes,MWCNTs)和铂纳米颗粒(Pt-nano)的电流型L-乳酸生物传感器。将Sol-gel膜覆盖在L-乳酸氧化酶(L-lactate oxidase,LOD)和MWCNTs/Pt-nano修饰的电极表面。实验结果表明:传感器的最佳工作条件为:检测电压0.5V;缓冲液pH6.4;检测温度25℃。此传感器的响应时间为5s,灵敏度是6.36μA/(mmol/L)。连续检测4星期其活性仍保持90%,线性范围为0.2～2.0mmol/L,且抗干扰能力强。在实际血样的检测中,此传感器与传统的分光光度法具有很好的一致性。 |
| 关 键 词： | 生物传感器 L-乳酸 溶胶-凝胶 铂纳米颗粒 多壁碳纳米管 |
Amperometric L-lactate Biosensor Based on Sol-Gel Film and Multi-walled Carbon Nanotubes/Platinum Nanoparticles Enhancement |
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| HE Xiao-Rui,YU Jing-Hua,GE Shen-Guang,ZHANG Xiu-Ming,LIN Qing,ZHU Han,FENG Shuo,YUAN Liang,HUANG Jia-Dong.Amperometric L-lactate Biosensor Based on Sol-Gel Film and Multi-walled Carbon Nanotubes/Platinum Nanoparticles Enhancement[J].Chinese Journal of Analytical Chemistry,2010,38(1). | |
| Authors: | HE Xiao-Rui YU Jing-Hua GE Shen-Guang ZHANG Xiu-Ming LIN Qing ZHU Han FENG Shuo YUAN Liang HUANG Jia-Dong |
| Institution: | College of Chemistry and Chemical Engineering1;College of Quan-cheng2;College of Medicine and Life Science3;University of Jinan;Jinan 250022 |
| Abstract: | An electrochemical L-lactate biosensor was fabricated by combining Platinum nanoparticles (Pt-nano) with multi-walled carbon nanotubes(MWCNTs).L-lactate oxidase(LOD) was immobilized on the surface of the glassy carbon electrode(GCE) modified with MWCNTs and Pt-nano.The surface of resulting LOD/MWCNTs/Pt-nano electrode was covered by a thin layer of sol-gel to avoid the loss of LOD and to improve the anti-interference ability.The cyclic voltammetric results indicated that MWCNTs/Pt-nano catalyst displayed a ... |
| Keywords: | Biosensor L-lactate Sol-gel Platinum nanoparticles Multi-walled carbon nanotubes |
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