Development of a photodiode array biochip using a bipolar semiconductor and its application to detection of human papilloma virus |
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Authors: | Taek Jin Baek Pan Yun Park Kwi Nam Han Ho Taik Kwon Gi Hun Seong |
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Institution: | (1) Department of Applied Chemistry, Hanyang University, Sa-1 dong 1271, Ansan, 425-791, South Korea;(2) 8F-8, Hightech Center, Gyeonggi Technopark, Celltek, Co., Ltd., Ansan, 426-170, South Korea |
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Abstract: | We describe a DNA microarray system using a bipolar integrated circuit photodiode array (PDA) chip as a new platform for DNA
analysis. The PDA chip comprises an 8 × 6 array of photodiodes each with a diameter of 600 μm. Each photodiode element acts
both as a support for an immobilizing probe DNA and as a two-dimensional photodetector. The usefulness of the PDA microarray
platform is demonstrated by the detection of high-risk subtypes of human papilloma virus (HPV). The polymerase chain reaction
(PCR)-amplified biotinylated HPV target DNA was hybridized with the immobilized probe DNA on the photodiode surface, and the
chip was incubated in an anti-biotin antibody-conjugated gold nanoparticle solution. The silver enhancement by the gold nanoparticles
bound to the biotin of the HPV target DNA precipitates silver metal particles at the chip surfaces, which block light irradiated
from above. The resulting drop in output voltage depends on the amount of target DNA present in the sample solution, which
allows the specific detection and the quantitative analysis of the complementary target DNA. The PDA chip showed high relative
signal ratios of HPV probe DNA hybridized with complementary target DNA, indicating an excellent capability in discriminating
HPV subtypes. The detection limit for the HPV target DNA analysis improved from 1.2 nM to 30 pM by changing the silver development
time from 5 to 10 min. Moreover, the enhanced silver development promoted by the gold nanoparticles could be applied to a
broader range of target DNA concentration by controlling the silver development time.
Figure An optical image of the PDA chip and target DNA detection through silver enhancement
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. |
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Keywords: | Biochips Photodiode array Human papilloma virus Gold nanoparticles DNA detection Silver enhancement |
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