Determination of myo-inositol in a flow-injection system with co-immobilized enzyme reactors and amperometric detection |
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Authors: | Bo Olsson György Marko-Varga Lo Gorton Roger Appelqvist Gillis Johansson |
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Institution: | Department of Analytical Chemistry, University of Lund, P.O. Box 124, S-221 00 Lund Sweden |
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Abstract: | A selective and sensitive flow-injection system for the determination of myo-inositol (hexahydroxycyclohexane) is described. Inositol dehydrogenase, IDH, lactate dehydrogenase, LDH, and lactate oxidase, LOD, are co-immobilized on porous glass and used in a packed-bed enzyme reactor. myo-Inositol reacts to produce an equivalent amount of hydrogen peroxide, which oxidizes hexacyanoferrate(II) to hexacyanoferrate(III) in a second reactor containing immobilized peroxidase. The hexacyanoferrate(III) is then detected amperometrically at 0 mV vs. SCE in a flow-through detector. The system responds linearly to injected samples of myo-inositol (25 μl) in the concentration range 1–300 μM. The maximum throughput was 90 samples per hour. The IDH/LDH/LOD reactor was stable for at least 5 weeks. |
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