Determination of methylglyoxal in mouse blood by liquid chromatography with fluorescence detection |
| |
| Authors: | Masatoshi Yamaguchi Shuuji Hara Masaru Nakamura |
| |
| Institution: | Faculty of Pharmaceutical Sciences, Fukuoka University, Nanakuma, Johnan-ku, Fukuoka 814-01 Japan |
| |
| Abstract: | A highly sensitive and rapid liquid chromatographic method for the determination of methylglyoxal in mouse blood is described, based on the precolumn conversion of methylglyoxal to a highly fluorescent 3-methyl-6,7-methylenedioxyquinoxaline by reaction with 1,2-diamino- 4,5-methylenedioxybenzene. The method is applied to the determination of methylglyoxal (0.1- 104 pmol) in 5 μ1 of blood. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
